THE CIRCUMFUSION SYSTEM FOR MULTIPURPOSE CULTURE CHAMBERS
نویسندگان
چکیده
منابع مشابه
The Circumfusion System for Multipurpose Culture Chambers
A self-contained mechanical system for circulating nutrient fluid through 12 tissue culture chambers is described in detail. This system utilizes nonperforated cellophane membranes in the chambers which separate the circulating nutrient from the tissue culture environments. The nutrient, therefore, is dialyzed through the cellophane of each chamber; some cell products are retained in the microe...
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A new technique for the cultivation of living tissues in the multipurpose culture chamber is described. This procedure employs strips of cellophane as the agent for anchoring tissue explants to the coverslip walls of the chamber and disposes of the time-honored plasma-clot technique. The primary advance embodied in this procedure lies in the fact that cells emigrating from so-cultured explants ...
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This paper describes a knowledge-based useroriented interface system which is unique in its combined use of data base, graphics, and AI technologies. All of the data presented to the user, as well as all of the data input by the user, is maintained and used as abstract “objects” using an AI object-oriented knowledge representation approach. However, the system employs a relational data base as ...
متن کاملII. The Protracted Maintenance of Differentiation of Fetal and Newborn Mouse Liver in Vitro
The circumfusion system is a complex in vitro pumping unit incorporating 12 multipurpose culture chambers through which a serum-supplemented fluid nutrient is rccirculatcd at a rate of 4.5 ml/min per chamber. This system was used to study the diffcrcntiative responses of fetal and newborn mousc liver cxplants placed in the serum-free environment formed betwccn thc shccts of unperforatcd celloph...
متن کاملThe Protracted Maintenance of Differentiation of Fetal and Newborn Mouse Liver in Vitro
The circumfusion system is a complex in vitro pumping unit incorporating 12 multipurpose culture chambers through which a serum-supplemented fluid nutrient is rccirculatcd at a rate of 4.5 ml/min per chamber. This system was used to study the diffcrcntiative responses of fetal and newborn mousc liver cxplants placed in the serum-free environment formed betwccn thc shccts of unperforatcd celloph...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1968
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb.39.2.430